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The purpose of this paper is to establish criteria that could guide the diagnosis, prognosis and treatment of canine mammary neoplasias. It was elaborated during the Mammary Pathology Meeting: Diagnosis, Prognosis and Treatment of the Canine Mammary Neoplasia, held on November 6th and 7th, 2010 in Belo Horizonte – MG, Brazil. Academics from several regions of Brazil were present and contributed to this work. After three years, a new discussion was found necessary in order to address important questions: 1 – Have Brazilian DVMs applied the consensus? 2 – What were the main difficulties in applying the consensus? 3 – What were the obtained results? 4 – What were the main differences among the various oncology services/groups? 5 – How could the criteria be improved and uniformed? A spreadsheet that allowed data collection and an abstract was submitted by each oncology service/group from various parts of the country. Based on the abstracts we identified the main differences in diagnosis and therapeutic conducts among the groups. These differences have guided the discussions of the II Mammary Pathology Meeting and the publication of a second consensus that has been revised and updated. The II Mammary Pathology Meeting: Diagnosis, Prognosis and Treatment of the Canine ammary Neoplasia, was held on December 9th, 10thand 11th, 2013 in Belo Horizonte – MG, sponsored by the Laboratory of Comparative Pathology of the Federal University of Minas Gerais (UFMG), with the support of the Brazilian Association of Veterinary Pathology (ABPV) and Brazilian Association of Veterinary Oncology (ABROVET). Academics from several regions of Brazil were present and contributed to this work.
Key Words: mammary neoplasias, dogs, mammary gland, veterinary oncology.
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Suppementary material: SUPPEMENTARY-MATERIAL-20881_2014_7_31_11_2
v.7, n.2, 2
In this research we studied the alterations in the muscles and myotendinous junction of dogs with muscular dystrophy. Gastrocnemius muscle (GM) and myotendinous junction (MTJ) samples of 3 controls and 6 dystrophic dogs were subjected to histopathological and immunohistochemical assays. Histopathological/histomorphometric analysis showed that the lesions in the GM were more pronounced and showed the highest percentage of Fiber Type II (FTII) and low values for the minimum diameters and areas of Fiber Type I (FTI) and FTII than those in the MTJ. FTII in dystrophic MTJ was morphologically similar to the controls. There was a significant difference (p < 0.07) in the MHCI antigen compared to the control. We conclude that the preservation of the morphological features of the MTJ can be directly related to a better stabilization of FTII, lower expression of the MHCI complex, and less cytotoxic activity of CD8.
Key Words: dogs, miopathy, Duchenne muscular dystrophy, immunohistochemistry, canine muscular dystrophy.
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A 4-year-old gray brocket deer was submitted to bilateral orchiectomy for enlargement of the right testicle, which measured 10 x 7 x 6 cm. The testicular tumor consisted of multiple cysts containing pilosebaceous brown material and translucent mucin. Histologically, the cysts showed keratinized stratified squamous epithelium over dermal tissue with hair follicles, sebaceous and sweat glands; pseudostratified cylindrical ciliated epithelium with goblet cells, ciliated columnar epithelium and secretory columnar epithelium. Among cysts, disorganized areas of adipose tissue, arteries, nerves, neurons, smooth muscle, dense connective tissue and cartilage tissue were observed. Epithelial and mesenchymal tissues were found well differentiated. The macro and microscopic examination of the testis allowed the diagnosis of benign cystic teratoma.
Key Words: wildlife diseases, gray brocket deer,benign cystic, teratoma, testis.
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Klebsiella spp. is a genus of the gram-negative Enterobacteriaceae family. Found in many environments, they are part of the natural microflora of soils, effluents, surface water, and in some plants. As an opportunistic agent, Klebsiella spp. mainly attacks subjects hospitalized with severe diseases as well as many fragile animals. This paper reports the occurrence of the infection Klebsiella pneumoniae in a nishikigoi carp, Cyprinus carpio, widely used as ornamental fish in aquaria, feeding and pituitary gland for the induction of reproduction. In a closed system for the domestic cultivation of ornamental fish there was found in carp an injury indicative of bacterial action. For confirmation and isolation of the possible pathogen, a tissue sample from a lesion was triturated and plated on Blood Agar, and incubated at 37° C for 24 hours. To identify the strain, biochemical tests were made based on manual methods described in the literature. In accordance with the phenotypic and biochemical tests conducted, Klebsiella pneumoniae was found to be the organism present in the carp lesion.
Key Words: aquaculture, ornamental fish, public health.
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An adult male Zebra finch (Taeniopygia guttata) with blood in the feces was examined for an internal mass in the abdominal caudal region near to the cloaca. During the necropsy, a large tumor mass around the large intestine was observed. The histopathology was suggestive of smooth muscle tumor and the immunohistochemistry analysis was performed for vimentin, desmin, SMA and KIT for the identification of the tumor histogenesis. The results were consistent with intestinal leiomyosarcoma.
Key Words: avian neoplasm, immunohistochemistry, histopathology, avian pathology, leiomyosarcoma.
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Lymphoma is the most prevalent neoplasia in dairy cattle. The etiology can be viral in animals affected by bovine leukemia virus (BLV) orbe classified as primary. Lymphoma can affect several organs and according to the system involved, the clinical signs could manifest themselvesin different ways. These tumors can be classified through macroscopic characteristics, histology and immunostaining. This classification can be used to predict prognosis and response to therapyThe aim of this case report was to immunostain and classify the tumor, for which anti-CD4, anti-CD8, anti-CD79 and anti-CD3 markers were used in addition to histopathological findings, in order to classify the tumor. The tumor was positive only for anti-CD3 marker, indicating that it is a tumor of young cells and, in association with histopathology and hematological data, it can be concluded that spleen neoplasia is lymphocyte lymphoma originated from a lymphocytic leukemia.
Key Words: immunophenotyping, bovine, CD3, lymphocytosis.
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A Rock Pigeon (Columba livia) submitted for necropsy had an oral white-yellow mass with a friable surface and putrid odor. Impression smears were performed and tissue samples were collected for histopathology. Cytopathological analysis revealed numerous pyriform protozoa, compatible with Trichomonas gallinae. Protozoans were not evident within the lesions by histopathology after staining the samples with Hematoxylin and Eosin or Gomori methenamine silver (GMS) stain. We conclude that impression smears from avian oral trichomoniasis suspects, even during post-mortem evaluation, can be a useful technique for the diagnosis of this disease.
Key Words: Trichomonas gallinae, avian pathology, cytopathology, Columba livia.
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A 14 year-old pregnant mare developed poor body condition (2/5) and diarrhea while grazing in kikuyu grass Pennisetum clandestinum. Clinical findings included bulging of nasal and maxillary bones and mouth lacerations associated to spicules in the surface of molars and premolars. Teeth were easily fragmented and fell off. Necropsy findings included severe softening of the maxillary and mandibular bones. The mandible was easily breakable near to the masseter´s fossa. The parathyroid gland was increased in size. Histological findings were characterized by multiple irregular and very thin bone spicules in the bone tissue, most of them with microfractures, severe osteoclasts-mediated bone resorption and fibrous connective tissue proliferation. Hyperplasia and hypertrophy of parathyroid chief cells with cytoplasmic vacuolization were also observed. In conclusion, maxillofacial fibrous osteodystrophy caused by nutritional secondary hyperparathyroidism developed in a pregnant mare while grazing kikuyu grass for long periods of time without appropriate supplements. Kikuyu grass is characterized by high levels of oxalic acid that forms insoluble oxalates responsible for low calcium absorption in the small intestine and the progress of hyperparathyroidism.
Key Words: nutritional hyperparathyroidism, Pennisetum clandestinum, oxalates, parathyroid gland, equine.
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November 29, 2017
November 29, 2017
November 29, 2017
November 29, 2017