v.9, n.1, 1
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v.9, n.1, 2
Renal involvement in Canine Monocytic Ehrlichiosis (CME) has been demonstrated in chronic cases without histopathological classification of patterns of glomerulopathy. Thus, in this study we proposed to evaluate the histopathological pattern, focusing on the types of glomerulonephritis in kidneys of dogs naturally infected with Ehrlichia canis.Twelve dogs naturally infected with E. canis and six healthy dogs were used. After clinical evaluation and diagnostic confirmation, the animals were euthanized and kidney fragments were obtained for histopathological examination, including hematoxylin and eosin (HE), Masson’s trichrome, periodic acid-Schiff (PAS), periodic acid-methenamine silver (PAMS) and Congo red stain. Histopathological analysis of dogs with CME demonstrated that major lesions were present in the glomerulus and tubulointerstitial region in 100% of cases. The type of glomerular injury was membranoproliferative in 83.33%, and proliferative in 16.67%. Interstitial nephritis was present in the cortical region of all infected dogs. Minimal to marked lymphohistioplasmacytic inflammatory infiltrate was present in the interstitial, perivascular and periglomerular areas. Differential count of inflammatory cells indicated that lymphocytes predominated in comparison to plasma cells and histiocytes. Interstitial fibrosis and hyaline casts of lower intensity were observed. There was a negative correlation between serum albumin levels and severity of glomerulopathy. In conclusion, membranoproliferative glomerulopathy and interstitial nephritis are the major renal lesions in CME. It has been suggested that the presence of inflammatory infiltrates rich in lymphocytes indicates participation of these infiltrates in the immunopathogenesis of renal lesion in dogs with CME. Furthermore, hypoalbuminemia also seems be a marker of renal damage in dogs infected with E. canis.
Key words: dog, Ehrlichia canis, kidney, histopathology.
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v.9, n.1, 3
Oil adjuvants in combination with inactivated vaccines against several avian diseases are widely used. In spite of some advantages of oil adjuvants, a local tissue reaction has been reported in some animals. In this report, about 30 birds of a flock of pigeons with 40 birds, developed scattered small to large nodules about 5 mm to 2 cm in diameter in subcutaneous tissues and pectoral muscles after intramuscularly injection of an oil-emulsified (ISA-70) Newcastle disease vaccine. Histopathologic examination showed granulomatous and cystic reactions in the site of injection. Bacteriological examinations as well as fresh smears stained with Ziehl-Neelsen, Brown-Brenn Gram and PAS stains were void of bacteria or fungi. It may be prudent to consider the potential for induction of adverse reaction of vaccine formulations containing mineral oils as adjuvant in pigeons.
Key words: ISA-70, vaccine reactions, granulomatous and cystic lesions, pigeon.
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v.9, n.1, 4
A free living female adult of Myrmecophaga tridactyla was captured in an area of deforestation in Salta Province of northwest Argentina, for subsequent relocation in Iberá Park. Three days after entering the quarantine it died with clinical signs of anorexia and ascites. It presented severe hypochromic microcytic anemia, hypoproteinemia and increase of CPK, AST and LDH on blood analysis. Grossly, there were ascites, hydrothorax, pulmonary congestion and severe catarrhal hyperplasic gastritis. The gastric mucosa was diffusely hyperemic with two clusters of numerous Physaloptera magnipapilla attached in the fundic region. Fundic mucosa was irregular with convoluted surface and covered with abundant brown-yellow catarrhal exudates. Microscopically, the gastric mucosa had well described punctate areas (pit-like structures) at points of P. magnipapilla attachment. Additionally, there were mucous hyperplasia of gastric glands and focal fibrosis with scarce infiltration of plasma cells, lymphocytes, multinucleated giant cells and eosinophils in the underlying lamina propria. This report describes a case of anemia and hyperplastic gastritis due to Physaloptera magnipapilla parasitism in a free-living Myrmecophaga tridactyla.
Key words: wildlife diseases, Physaloptera magnipapilla, hypochromic microcytic anemia, hyperplastic catarrhal gastritis.
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v.9, n.1, 5
An adenoma of the uterine tube in a 14-year-old, mixed breed, female dog with history of abdominal distention is described. Ultrasonographic examination revealed a 15 x 20 cm anechoic mass in the left caudal region of the abdominal cavity. Laparotomy was performed and the mass, within the left ovarian bursa, was surgically excised by ovariohysterectomy. Grossly, the left ovarian bursa was markedly increased in size (7 x 15 x 20 cm) and contained approximately 300ml of serosanguineous fluid that surrounded a 5 x 13 x 17 cm in diameter, red, papilliform, and soft mass. Histologically, the mass was characterized as an epithelial neoplastic proliferation arranged in a papilliform pattern. The numerous papillae were lined by a single layer of well-differentiated columnar ciliated cells, with occasional cuboidal cells, and supported by a dense fibrovascular stroma. Columnar cells contained abundant, slightly vacuolated and granular cytoplasm. The granules were located predominantly in the apical surface and were Alcian blue and periodic acid-Schiff positive. Nuclei were round and polarized in the apical surface of the cell. Neoplastic cells were positive for pan-cytokeratin and vimentin. The diagnosis of adenoma of uterine tube was based on the anatomic location, and histological, immunohistochemical and ultrastructural findings.
Key words: neoplasms, dogs, female reproductive system, adenoma, pathology
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v.9, n.1, 6
Dysgerminoma and granulosa cell tumor are neoplasms of the ovary of domestic animals, originating from germ cells and sex-cords cells, respectively. These neoplasms generally occur unilaterally, and usually as a single neoplasm. This report describes a case of a dysgerminoma in the right ovary and a granulosa cell tumor in the left ovary in a six years old bitch. An Akita and Siberian Husky crossbred bitch was admitted at the Veterinary Hospital (Universidade Vila Velha), with a history of purulent vaginal discharge, irregular estrous cycle, fatigue, polydipsia and polyuria. Ultrasound exam demonstrated bilateral ovarian neoplasms, and the animal was subjected to ovariohysterectomy. Macroscopic and microscopic features found in right and left ovaries were typical of dysgerminoma and granulosa cells tumor Sertoli-like, respectively. The uterus had cystic endometrial hyperplasia associated with mild metritis. Ovarian neoplasms occurring bilaterally are uncommon especially with different cell origins and distinct histopathological patterns in each ovary as described in this report.
Key words: ovary, ovarian neoplasm, female reproduction, metritis, hyperestrogenism.
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v.9, n.1, 7
A 5-year-old African hedgehog (Atelerix albiventris) was presented to the veterinarian with history of anorexia and progressive weight loss. On clinical examination the mucous membranes were pale, and the skin exhibited extensive alopecia with crusting in all four limbs and tail. A large subcutaneous mass was palpated on the left lateral femur which subsequently was diagnosed histopathologically as a squamous cells carcinoma. The owner declined further tests and the patient returned home where it continued to deteriorate and finally died 90 days after initial presentation. The carcass was submitted for postmortem examination. Necropsy finding included an enlarged spleen with rounded borders and meaty pulp, hyperplastic bone marrow and multiple white foci in both kidneys. Tissues were submitted for cytology, histopathology and electron microscopy. Splenic cytology revealed a monomorphic population of granulocytes with cellular atypia which were most consistent with neoplastic eosinophils. Similar myeloid cells were also seen histologically in kidneys, liver, intestine, heart, skin and brain. The bone marrow was completely effaced with similar cellular infiltrates. Luna stain for eosinophils was positive in all tissues. Electron microscopy showed that neoplastic cells had granules and electron-lucent crystalloid characteristic of eosinophils. Based on these finding chronic eosinophilic leukemia was diagnosed, and to our knowledge, eosinophilic leukemia in hedgehogs is rarely reported in the literature.
Key words: wildlife diseases, neoplasia, eosinophilic leukemia, African hedgehog, Atelerix albiventris.
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