v.10, n.1, 1
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v.10, n.1, 2
A total of 290 cases of tarsal gland adenomas and epitheliomas from canine patients were analyzed to determine the frequency and to better characterize their histopathological features. Tissue samples were examined in two veterinary pathology laboratories in Curitiba, Brazil, which receive samples from all over the country. Tarsal epitheliomas were significantly more frequent than tarsal adenomas (77.9% and 20.7%, respectively). In four cases (1.4%), the lesion contained both types of neoplasms. A total of 39 breeds were represented. The most common breeds were: English cocker spaniel (12.4%), poodle (10.3%) and Labrador retriever (9.3%). Mixed breed dogs represented 13.1% of the total. The upper eyelids were most commonly affected (65.2%), males and females were equally represented and there did not appear to be a predilection for either side of the face. Mitotic figures were most common in epitheliomas. The pigmented subtype was the most common form of epithelioma (59.7%). Whereas within the adenomas, the classic subtype (without inflammation or pigmentation) was the most common form (61.7%); and the inflamed subtype was also highly represented (20.0%).
Key words: adenoma, epithelioma, histopathology, tarsal gland, dog.
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v.10, n.1, 3
The aim of the present study was to investigate the effects of intraportal administration of hypothermic hydrogen-rich saline solution (HRSS) on hepatic ischemia/reperfusion (I/R) injury. Thirty rats were divided equally into six groups; 1) sham, no I/R or transfusion, 2) I/R injury (60 minutes ischemia + 120 minutes reperfusion, 3) I/R injury + normal saline 24°C, 4) I/R injury + normal saline 4°C, 5) I/R injury + HRSS 24°C, 6) I/R injury + HRSS 4°C. In groups 3-6, 1 mg/kg normal saline (NS) and/or HRSS were administered into the vein of the left lateral and median lobes of the liver (upper the site of clumping) 10 minutes before finishing of ischemic period. The harvest time points were at 2 hours post reperfusion in all groups. Cell death, sinusoidal dilatation, congestion, hemorrhage, and neutrophil infiltration were observed in I/R group, while these histopathological changes were attenuated in the hypothermic HRSS administrated groups (P < 0.01). The level of alanine aminotransferase, aspartate aminotransferase, malondialdehyde, interleukin 6, tumor necrosis factor a, and caspase-3 were increased significantly by I/R injury and hypothermic HRSS administration reduced all these markers (P < 0.01). SOD level was low in I/R group whereas it tended to increase in the hypothermic HRSS administrated groups (P < 0.01). The present study demonstrated that hypothermic hydrogen-rich saline solution effectively protected the hepatic tissue against cellular injury and organ dysfunction through the mechanisms that decrease the effect of oxidative stress, inflammation, apoptosis and necrosis.
Key words: hydrogen-rich saline solution, hypothermia, ischemia/reperfusion injury, liver, rat.
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v.10, n.1, 4
The aim of this study was to describe patterns of histopathological recognition of the cresty neck in Horses in Spain. A total of 250 horses were studied in Andalusia and Extremadura, Spain. Seventy-six percent of horses present cresty neck. The damage of the cresty neck in horses was categorized as Grade 0 – Muscle fibers are observed, no fat vacuoles are observed (24% of the horses). Grade 1 – Scarce adipose deposit was observed. An unaltered muscle tissue is observed (21% of the horses). Grade 2 – fat vacuoles are evident in muscle tissue intermyofibrillar space and prone to coalescence (23% of the horses). Grade 3 – Abundant fat vacuoles in the intermyofibrillar space, with tendency to coalesce, and low fat infiltration in muscle tissue (moderate lipomatosis) (16% of the horses). Grade 4 – Abundant fat vacuoles in the intermyofibrillar space, with tendency to coalesce, and fatty infiltration in muscle tissue (marked lipomatosis) (8% of the horses). Grade 5 – Only fat vacuoles are observed, without muscle tissue (severe lipomatosis) (8% of the horses). The results for desmin antibody (1: 100 dilution) was positive (++) in grades 0-2, and negative (-) in grades 3-5. These results suggest that as fat/lipomatosis infiltration increases (progresses in grades 3, 4 and 5), the intercellular space (intermyofibrillar) increases and therefore the cell cytoskeleton is lost, with loss of the bands Z, so the negative response to this antibody. In conclusion, we describe histopathological pattern recognition of cresty neck in horses in Spain.
Key words: biopsy, cresty neck, histopathology, horses.
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v.10, n.1, 5
The goal of this case report is to describe a salivary gland adenocarcinoma in a free ranging Sphiggurus villosus. A orange-spined hairy dwarf porcupine (S. villosus) was referred to the Wildlife Medicine Service at the Veterinarian Hospital of the Universidade Estadual Paulista (Jaboticabal, São Paulo, Brazil), with a mass in the lateral region of the face. Radiographs highlighted swelling in the soft tissues, measuring 7 cm x 9 cm, without bone involvement. The physical examination and radiographs were consistent with a neoplastic process. The animal was submitted to surgical excision of the mass. Microscopically, the tumor was composed of a highly cellular proliferation of epithelial cells arranged predominantly in tubular pattern, associated with moderated fibrous stroma, poorly demarcated, non-encapsulated and with infiltrative growth. These findings were consistent with salivary gland adenocarcinoma. To the authors’ knowledge, this is the first report of a salivary gland adenocarcinoma in a wild life orange-spined hairy dwarf porcupine.
Key words: rodentia, wildlife, neoplasia, surgery, clinical-histological diagnostic.
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v.10, n.1, 6
A 12 year-old mixed breed female cat was present with signs of dyspnea, cyanosis and drooling, followed by sudden death. At necropsy we observed nodules measuring 0.5 x 0.5 to 2.0 x 2.0 cm in the pulmonary parenchyma showed multifocal atelectasis associated with discrete emphysema. The histopathological examination of lung masses revealed neoplastic epithelial cells compatible with bronchioloalveolar carcinoma (BAC). The immunohistochemical analysis of tumor cells showed positive labeling for pan-Cytokeratin, CK 7, CK 20 and TTF-1. According to macroscopic features, as well as histological and immunohistochemical findings, this tumor was diagnostic as BAC mixed subtype.
Key words: feline, lung, immunohistochemistry, neoplasia.
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v.10, n.1, 7
The African Hedgehog (Atelerix albiventris) has gained popularity as a pet in several parts of the world. This is a report of a concomitant finding of spontaneous mammary and uterine tumors in an adult female African Hedgehog. The animal presented abdominal masses and died suddenly. Upon necropsy, not only the mammary tumor mass, but also a uterine neoplasm was found. Formalin fixed and paraffin embedded tissues were submitted for histopathological examination. The mammary tumor was diagnosed as a solid carcinoma, and the uterine tumor as fibrosarcoma. By immunohistochemistry, the mammary tumor showed positivity for pancytokeratins and estrogen and progesterone receptors, while the uterine neoplasm exhibited positivity for vimentin. There was high cross-immunoreactivity between anti-human antibodies. The positivity for hormonal receptors antigens may represent a relationship between estrogen and progesterone levels and the development of mammary tumors, as in other mammals.
Key words: wild animals, exotic animals, cancer, histopathology, concurrent tumors, progesterone receptor, estrogen receptor, immunohistochemistry, diagnosis.
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