Comparative Analysis of Histopathological Alterations and Immunohistochemistry in Cattle for Diagnosis of Rabies

Authors

  • Akeel Bashir Beigh Department of Veterinary Pathology Author
  • Bhupinder Singh Sandhu Department of Veterinary Pathology Author
  • Charan Kamal Singh Department of Veterinary Pathology Author
  • Karan Bansal Veterinary Polytechnic & RRTC Author
  • Madhav Mugale Nilakanth Department of Veterinary Pathology Author
  • Naresh Kumar Sood Department of Veterinary Pathology Author

Keywords:

cattle, histopathology, immunohistochemistry, rabies

Abstract

Comparative analysis of histopathological alterations and immunohistochemistry was performed on 15 brain samples of cow. In the present study, brain tissues were positive in 9 cases (60.0%), using polyclonal antiserum by immunohistochemistry and 7 cases (46.60%) were found positive for rabies by demonstration of Negri bodies. Hundred neurons per case were observed for Negri bodies; number of Negri bodies in positive neurons was counted and a comparison of IHC and histopathology was done. Number of neurons positive for rabies virus antigen per 100 neurons (900 neurons) by IHC were more (665) than H& E staining (344) and average number of Negri bodies per neuron detected by IHC were more (2.97) than histopathology (1.52), therefore, IHC was found to be more sensitive than histopathology.

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Published

2013-03-30

Issue

Vol. 6 No. 1 (2013): BJVP

Section

Artigos

How to Cite

Beigh , A. B., Sandhu, B. S., Singh , C. K., Bansal , K., Nilakanth , M. M., & Sood, N. K. (2013). Comparative Analysis of Histopathological Alterations and Immunohistochemistry in Cattle for Diagnosis of Rabies. Brazilian Journal of Veterinary Pathology, 6(1), 6-10. https://bjvp.org.br/bjvp/article/view/139