Detection of reticuloendotheliosis virus by immunohistochemistry and in situ hybridization in experimentally infected chicken embryo fibroblasts

Authors

  • Vitor L.S.L. Santos Department of Pathology, College of Veterinary Medicine, University of Georgia Author
  • Susan Williams Poultry Diagnostic and Research Center, Department of Population Health, University of Georgia Author
  • Guillermo Zavala Poultry Diagnostic and Research Center, Department of Population Health, University of Georgia Author
  • Jian Zhang Department of Pathology, College of Veterinary Medicine, University of Georgia Author
  • Sunny Cheng Poultry Diagnostic and Research Center, Department of Population Health, University of Georgia Author
  • Renato L. Santos Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais Author
  • Corrie C. Brown Department of Pathology, College of Veterinary Medicine, University of Georgia Author

Keywords:

Immunohistochemistry, in situ hybridization, Reticuloendotheliosis, chicken, fibroblast

Abstract

Reticuloendotheliosis virus (REV) infection can result in immunosuppression, runting syndrome, high mortality, acute reticular cell neoplasia, or T- and/or B-cell lymphoma, in a variety of domestic and wild birds. Histopathological changes of reticuloendotheliosis (RE) are not sufficient to differentiate it from Avian Leukosis (AL) and Marek’s disease (MD). Currently there are no available diagnostic methods for detection of active REV infection. In order to develop immunohistochemistry (IHC) and in situ hybridization (ISH) assays for detection of REV active infections, experimentally inoculated formalin-fixed and paraffin embedded DF-1 chicken embryo fibroblasts were used as an infection model. IHC and ISH assays proved to be efficient for the detection of several REV strains, and to differentiate those strains from representative strains of the avian leukosis/sarcoma group of retroviruses (ALSV).  

Author Biographies

  • Vitor L.S.L. Santos, Department of Pathology, College of Veterinary Medicine, University of Georgia

    Department of Pathology, College of Veterinary Medicine, University of Georgia

  • Susan Williams , Poultry Diagnostic and Research Center, Department of Population Health, University of Georgia

    Poultry Diagnostic and Research Center, Department of Population Health, University of Georgia

  • Guillermo Zavala , Poultry Diagnostic and Research Center, Department of Population Health, University of Georgia

    Poultry Diagnostic and Research Center, Department of Population Health, University of Georgia

  • Jian Zhang, Department of Pathology, College of Veterinary Medicine, University of Georgia

    Department of Pathology, College of Veterinary Medicine, University of Georgia

  • Sunny Cheng , Poultry Diagnostic and Research Center, Department of Population Health, University of Georgia

    Poultry Diagnostic and Research Center, Department of Population Health, University of Georgia

  • Renato L. Santos , Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais

    Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais

  • Corrie C. Brown , Department of Pathology, College of Veterinary Medicine, University of Georgia

    Department of Pathology, College of Veterinary Medicine, University of Georgia

Downloads

Published

2009-05-30

Issue

Vol. 2 No. 1 (2009): BJVP

Section

Artigos

How to Cite

Santos, V. L., Williams , S., Zavala , G., Zhang, J., Cheng , S., Santos , R. L., & Brown , C. C. (2009). Detection of reticuloendotheliosis virus by immunohistochemistry and in situ hybridization in experimentally infected chicken embryo fibroblasts. Brazilian Journal of Veterinary Pathology, 2(1), 29-34. https://bjvp.org.br/bjvp/article/view/25