Quantification of the collagen fibers of healthy and neoplastic mammary tissue in cats by the Picrossirius Red histochemical method

Authors

  • Mariana Fernandes Jorge Faculty of Veterinary Medicine and Animal Science, Universidade Estadual Paulista (UNESP) - Botucatu Author
  • Ana Cláudia Patrocínio Faculty of Electrical Engineering, Federal University of Uberlândia Author
  • Alessandre Hataka Faculty of Veterinary Medicine and Zootechnics, Universidade Estadual Paulista (UNESP) - Botucatu Author
  • Júlio Lopes Sequeira Faculty of Veterinary Medicine and Zootechnics, Universidade Estadual Paulista (UNESP) - Botucatu Author

DOI:

https://doi.org/10.24070/bjvp.1983-0246.v14i1p18-23

Keywords:

carcinoma, feline, breast density, extracellular matrix, breast cancer

Abstract

Although feline mammary carcinoma is not the most prevalent among the species, its aggressive behavior represents a
low life expectancy, compared with most undifferentiated types of breast cancer. Tissue stiffness induced by the accumulation
of collagen fibers is related to a risk factor for carcinogenesis in healthy women and aggressiveness in those with breast cancer,
which can also occur in cats. The objective of this work is to identify the relationship between stromal collagen density and
aggressiveness of mammary carcinoma in cats, according to the peripheral and central tissue distribution by the Picrossirius Red
histochemical method. Image.J® and MatLab® software were used for digital image processing. The mean values of kurtosis
and entropy attributes were grouped into a control group, and low and high-grade carcinoma groups, analyzed with one-way
ANOVA and Bonferroni’s multiple comparison test (p <0.01). Interpretation of stromal dynamics is important to evaluate both
central and peripheral locations. According to entropy, there was a significant increase in the peripheral density in the carcinoma
groups in relation to the control group, which can be justified by blood support. The same can be said of the central region, with a
significant gain in collagen fibers from the tumors, indicated by kurtosis. The results suggest the presence of increases in stromal
density in mammary carcinomas of cats, regardless of their graduation, and occurring in both regions.

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Published

2021-03-30

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Artigos

How to Cite

Jorge, M. F., Patrocínio, A. C., Hataka, A., & Sequeira, J. L. (2021). Quantification of the collagen fibers of healthy and neoplastic mammary tissue in cats by the Picrossirius Red histochemical method. Brazilian Journal of Veterinary Pathology, 14(1), 18-23. https://doi.org/10.24070/bjvp.1983-0246.v14i1p18-23