v.11, n.1, 1
Original Full Paper
Immunohistochemical expression of Galectin-3 in canine tumors
Thiago H. M. Vargas, Lidia H. Pulz, Ricardo F. Strefezzi
Abstract
Galectin-3 (Gal-3) is a protein expressed by both normal and neoplastic cells. It participates in several biological processes such as cell proliferation, cell adhesion, apoptosis, tissue remodeling, and angiogenesis. Although it is known to serve as a valuable prognostic marker in several types of human cancer, there are few reports about its applicability as a marker in the veterinary oncology literature. The aim of the present study was to characterize Gal-3 expression in different types of canine tumors. Fifty-three tissue samples from 22 histologically different types of canine tumors were immunohistochemically evaluated for Gal-3 expression. Variations in the percentage of Gal-3-positive cells, localization of Gal-3 protein, and percentage of Gal-3-positive fibroblasts were observed. These preliminary results showed variable expression of Gal-3 among canine tumors. Further studies are needed in order to investigate the potential of this protein as a prognostic marker and a therapeutic target.
Key words: canine, Galectin-3, immunohistochemistry, cancer, prognosis.
Download full article: v11 n1 – 1
DOI: 10.24070/bjvp.1983-0246.v11i1p01-06
v.11, n.1, 2
Case Report
Granulomatous rhinitis in a case of feline leishmaniasis
Alexandre Arenales, Camila Eckstein, Jaqueline Azevedo, Gisele M. S. Reginaldo, Valéria M. F. Lima, Daniela B. Rozza, Renato L. Santos
Abstract
This is a case report of leishmaniasis with granulomatous rhinitis in a 2-year-old domestic cat. Clinically, an obstruction in the right nostril was observed, with cytopathological examination positive for Leishmania sp. Euthanasia was performed, and multinodular, diffuse and smooth pinkish lesions were observed in the mucosa of the nasal cavity. Microscopically, nodular lesions corresponded to an intense granulomatous inflammation with macrophages containing large numbers of Leishmania sp. amastigotes. Macrophages containing amastigotes were also occasionally observed in the popliteal lymph node, skin, and spleen. Based on immunohistochemistry, amastigotes were confirmed as Leishmania sp. and Leishmania sp. DNA was also detected by qPCR in lymph nodes samples. Although it is an uncommon manifestation, leishmaniasis should be considered as a differential diagnosis in diseases affecting the nasal cavity in domestic felines.
Key words: immunohistochemistry, cat, nasal cavity, leishmaniasis.
Download full article: v11 n1 – 2
DOI: 10.24070/bjvp.1983-0246.v11i1p07-11
v.11, n.1, 3
Case Report
Hemosuccus pancreaticus in a dog: A rare case of gastrointestinal hemorrhage
Ana F. M. Botelho, Matheus V. L. Moreira, Camila Eckstein, Fabrício G. Melo, Letícia B. Oliveira, Cláudia E. V. Rocha, Cibele J. Santos, Anelise C. Nepomuceno, Natália M. Ocarino, Rogéria Serakides
Abstract
A 9-year-old, 5.5-kg (12.12-lb) spayed female Poodle was evaluated due to recurrent seizures and possible diabetes. The dog also had a history of anorexia, polyuria and polydipsia. Serum biochemistry results revealed increases in pancreatic amylase and glucose associated with decreased protein levels. Abdominal ultrasound suggested acute pancreatitis and/or a pancreatic tumor with intense peripancreatic vascularization. The clinical condition of the dog worsened despite medical treatment, and death occurred shortly thereafter. At necropsy, blood clots were found in the stomach and in the intestinal lumen, which was filled with undigested blood. The pancreas was moderately increased in size with an irregular surface and multifocal yellow firm areas. In the cranial portion of the pancreas, a single nodule, approximately 2 cm in diameter, was found. Histologic evaluation of the pancreas revealed chronic and acute lesions characterized by lymphohistiocytic pancreatitis, periductal fibrosis, degeneration of pancreatic islets and necrotizing and fibrinopurulent pancreatitis. The nodule was diagnosed as pancreatic adenocarcinoma. These clinical, imaging, and histopathologic findings were compatible with Hemosuccus pancreaticus. To the authors’ knowledge, this is the first report of Hemosuccus pancreaticus in a dog and it should be considered as a differential diagnosis for dogs with pancreatic disease and gastrointestinal bleeding.
Key words: dog, Hemosuccus, pancreas, pancreatitis.
Download full article: v11 n1 – 3
DOI: 10.24070/bjvp.1983-0246.v11i1p12-18
v.11, n.1, 4
Case Report
Metastatic submandibular acantholytic squamous cell carcinoma in a dog
Isabella C. Silva, Thais B. Sarandy, Natalia V. Tamiasso, Karina P. Aptekmann, Paulo S. C. Andrade Junior and Jankerle N. Boeloni
Abstract
A seven-year-old female mixed-breed dog was diagnosed with an uncommon histological variant of squamous cell carcinoma (SCC) with lymph node metastases. Therefore, the objective of this report was to describe the gross and microscopic findings of this uncommon SCC histological variant. Fine-needle aspiration cytology of the mass revealed a squamous cell carcinoma. On necropsy, the submandibular region revealed nodular, multifocal to coalescing, ulcerated masses with firm and fluctuant areas. Furthermore, enlarged submandibular and cervical lymph nodes with loss of normal architecture suggestive of metastasis were present. A definitive diagnosis of submandibular acantholytic squamous cell carcinoma was made upon histopathological evaluation. This variant is rare in humans and animals.
Key words: canine, epidermoid cell carcinoma, metastasis, pseudoglandular carcinoma.
Download full article: v11 n1 – 4
DOI: 10.24070/bjvp.1983-0246.v11i1p19-23
v.11, n.1, 5
Case report
Melanoma of unknown primary origin metastasizing to visceral organs in a sheep
Ahmad Oryan, Mohsen Ghane, Nasrollah Ahmadi, Yahya Daneshbod
Abstract
Malignant melanomas are quite uncommon in sheep and goats. A three-year-old native female sheep with a history of sudden death was referred for postmortem examination. Gross evaluations revealed multiple brown to black neoplastic masses with compact nodular appearance, involving lungs, mediastinal lymph nodes, liver and kidneys. Comprehensive physical examination of skin, all mucous membranes and mucocutaneous junctions revealed no evidence of concomitant pigmented lesions or dark discoloration. Microscopically, the masses were predominantly composed of round and polyhedral-shaped neoplastic cells of various sizes with abundant pale eosinophilic cytoplasm containing variable amounts of dark brown melanin pigment and peripherally located, oval to round, vesicular nuclei with prominent nucleoli. Immunohistochemically, the neoplastic cells displayed diffuse and positive expression for S100 protein, vimentin and melan-A. These histopathological and immunohistochemical findings were consistent with a final diagnosis of metastatic malignant melanoma, “epithelioid” type. To the author’ knowledge, metastatic melanoma without an identified primary origin has not yet been reported in the domestic animals.
Key words: sheep, malignant melanoma, visceral organs, histopathology, immunohistochemistry, melan A.
Download full article: v11 n1 – 5
DOI: 10.24070/bjvp.1983-0246.v11i1p24-27
v.11, n.1, 6
Case Report
Thromboembolic encephalitis secondary to bacterial valvular endocarditis in a red-billed curassow (Crax blumenbachii)
Andréia Vielmo, Ronaldo M. Bianchi, Fernando F. Argenta, Veronica M. Rolim, Gustavo G. M. Snel, Bruna Zafalon-Silva, Marcelo M. Alievi, Claudio S. L. de Barros, David Driemeier
Abstract
A case of bacterial (Pseudomonas aeruginosa) valvular endocarditis in a red-billed curassow is described. A traumatic fracture in one limb of the bird was considered the port of entry for the bacterium, followed by septicemia and seeding of the heart valve. Brain embolism resulting from detached thrombus fragments followed.
Key words: Cracidae, curassow, encephalitis, valvular endocarditis, Pseudomonas aeruginosa.
Download full article: v11 n1 – 6
DOI: 10.24070/bjvp.1983-0246.v11i1p28-31
v.11, n.1, 7
Case Report
Diffuse unilateral seminoma in a fighting rooster (Gallus gallus)
Nestor Ledesma, Adriana Méndez, José A. González, Elizabeth Morales
Abstract
The aim of this study was to report a diffuse unilateral seminoma in a 4-year-old fighting rooster (Gallus gallus) by considering the anatomic, pathological and ultrastructural features. A significant decline in the viability of the bird’s embryos was reported during the last year. The bird died with a marked abdominal distention. During necropsy, a firm ovoid neoplasm of approximately 9 cm long, 8 cm wide and 5 cm thick was found in the region adjacent to the pronephros, replacing the left testicle. The neoplasia was soft and encapsulated, and the cut surface showed extensive areas of haemorrhage interspersed with necrotic areas. Histologically, the neoplastic cells formed large mantles or cords with moderate and eosinophilic cytoplasm. The nuclei were pleomorphic, and some of them were central, while others were eccentric. Mitoses were scarce. Some important findings in the ultrastructural study were nuclei exhibited an elongated, electrodense, rope-like nucleoli and the presence of scarce intracytoplasmic glycogen. The anatomo-pathological and ultrastructural findings of the tumour were consistent with a seminoma. The ultrastructural study provided important support for the diagnosis, as other tumours were ruled out. The early diagnosis of malignant or benign testicular tumours in birds is the key for a timely treatment, as these tumours can metastasize or grow to such an extent that they compromise the lives of birds, as in the present case.
Key words: Gallus gallus, testis, seminoma, ultrastructure.
Download full article: v11 n1 – 7